논문투고

축산관련 논문을 투고한 자료를 모아 정보를 제공합니다.
(관련자료가 없는 성과년도는 표기되지 않습니다.)
출처 : 농촌진흥사업 종합관리시스템

글보기입니다.
논문명(한글) Expression and purification of ESBP in Escherichia coli, the extracellular protein derived from Bifidobacterium longum KACC 91563
논문명(영문) Expression and purification of ESBP in Escherichia coli, the extracellular protein derived from Bifidobacterium longum KACC 91563
예산사업명 축산시험연구
연차연구세부과제 비피더스균(KACC 91563)의 대량배양 및 유육제품 적용 연구
성과주관부서 국립축산과학원 축산물이용과
품목코드 축산 / 대가축 / 젖소
학술지명 Food Science of Animal Resources 주저자 송민유(국립축산과학원)
연도 2018 성과적용일 2019년09월
Family 5 extracellular solute-binding protein (ESBP) is a component of the extracellular vesicle (EV) secreted by Bifidobacterium longum KACC 91563. The property of ESBP that effectively alleviates food allergy symptoms via mast cell specific apoptosis was demonstrated in our previous work, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, B. longum KACC 91563 ESBP gene was cloned into the histidine-tagged (His-tag) pET-28a(+) vector, and the resulting plasmid was transformed into the Escherichia coli strain BL21(DE3). The transformed cells were used to grow and express the histidine-tagged ESBP and the expressed protein was purified using a Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the optimization process, two liters of transformed culture produced total of 7.15 mg of the recombinant ESBP proteins with molecular weights of approximately 60.0 kDa. This is the first study describing the production of ESBP using recombinant DNA technology. Establishment of large-scale production strategy for ESBP protein will contribute to the development a potential alternative treatment for allergies.
  • 트위터
  • 페이스북

담당자 : 기획조정과 전승일[063-238-7131]  ㅣ  갱신주기 : 변경시

자주찾는 정보

자주찾는 정보

원하시는 메뉴 5개 선택 후 저장하세요